Table 1 Average quality measures of assemblies based on data from Illumina MiSeq, ONT MinION, or both. IL = Illumina only, R9ONLR = R9ONT assemblies polished with ONT reads, R9ONLRSR = R9ONT assemblies additionally polished with Illumina short reads, R9UC = Unicycler assemblies based on R9ONT and Illumina, R10ONLR = R10ONT assemblies polished with ONT reads, R10ONLRSR = R10ONT assemblies polished additionally with Illumina short reads, R10UC = Unicycler assemblies based on R9ONT and Illumina
| Â | Name | #Baseparis | #Contigs | N50 bp | %GC | % Coverage reference genome |
|---|---|---|---|---|---|---|
Ba. anthracis | IL | 5.454.344 | 85,0 | 213.938 | 35,12 | 99,01 |
R9ONLR | 5.505.633 | 3,0 | 5.229.038 | 35,25 | 99,99 | |
R9ONLRSR | 5.506.307 | 3,7 | 5.228.860 | 35,25 | 99,98 | |
R9UC | 5.504.342 | 3,3 | 5.227.886 | 35,25 | 99,98 | |
R10ONLR | 5.489.536 | 2,8 | 5.228.731 | 35,26 | 99,69 | |
R10ONLRSR | 5.489.204 | 2,8 | 5.228.416 | 35,26 | 99,69 | |
R10UC | 5.504.352 | 3,5 | 5.202.007 | 35,25 | 99,98 | |
Ames Ancestor | 5.227.419 | 3,0 | Â | 35,24 | Â | |
Br. suis | IL | 3.306.237 | 34,0 | 184.611 | 57,23 | 98,82 |
R9ONLR | 3.328.684 | 2,0 | 1.962.140 | 57,21 | 99,34 | |
R9ONLRSR | 3.328.513 | 2,0 | 1.962.040 | 57,21 | 99,34 | |
R9UC | 3.329.933 | 4,0 | 1.943.040 | 57,21 | 99,29 | |
R10ONLR | 3.328.496 | 2,0 | 1.962.244 | 57,21 | 99,33 | |
R10ONLRSR | 3.328.496 | 2,0 | 1.962.075 | 57,21 | 99,33 | |
R10UC | 3.329.930 | 3,7 | 1.943.035 | 57,2 | 99,29 | |
1330 | 3.315.175 | 2,0 | Â | 57,25 | Â | |
F. tularensis | IL | 1.788.491 | 103,8 | 25.987 | 32,17 | 94,21 |
R9ONLR | 1.894.822 | 1,7 | 1.892.558 | 32,17 | 99,75 | |
R9ONLRSR | 1.894.774 | 1,7 | 1.892.510 | 32,17 | 99,75 | |
R9UC | 1.876.814 | 1,3 | 1.578.270 | 32,17 | 98,92 | |
R10ONLR | 1.892.497 | 1,0 | 1.892.376 | 32,16 | 99,75 | |
R10ONLRSR | 1.892.460 | 1,0 | 1.892.460 | 32,165 | 99,75 | |
R10UC | 1.871.521 | 1,5 | 1.423.518 | 32,17 | 98,65 | |
OSU18 | 1.895.727 | 1,0 | Â | 32,16 | Â |