Fig. 5

The second Huntiella a-factor pheromone locus, harboring up to three additional a-factor pheromone homologs. A Gene maps of the locus showing conservation of gene content with evidence for an inversion of the region between genes U2 and U3. This inversion appears to have occurred in the ancestor of H. savannae, H. decipiens, and H. bhutanensis, as the locus structure is shared by H. abstrusa, H. omanensis and the three unisexual species. Notably, H. decipiens and the three unisexual species lacked the a5 genes. Additionally, their a6 genes were truncated. This figure was drawn using data from BLASTn and tBLASTn analyses using the H. abstrusa homologs as queries and is not drawn to scale. The identity of the additional non-pheromone genes at this locus can be found in Table S3. B A zoomed in region of the gene maps from H. abstrusa and H. omanensis and the sequences from the H. abstrusa a6 protein and the H. omanensis a6 and a7 proteins. This shows that the gene is split in H. omanensis, with the H. omanensis a8 gene encoding a protein homologous to the N-terminal of the H. abstrusa a6 protein and the H. omanensis a6 gene encoding a protein homologous to the C-terminal of the H. abstrusa a6 protein