Fig. 2
HMBOX1 affected LPS-induced macrophage activation. Transfected RAW264.7 cells were plated at density of 2 × 105 cells/well in 6 plates with 2 mL DMEM and stimulated with LPS (10 ng/mL). (A) Decreased levels of cytokines TNF-α and IL-6 were observed in the HMBOX1-overexpressed group with 24 h LPS stimulation. (B) RAW264.7 cells were stimulated with LPS (10 ng/mL) for 3 h, then, neutral red solution (0.1%) was added and co-incubated with RAW264.7 cells for 1 h. Decreased phagocytosis ability was also observed in the HMBOX1-overexpressed RAW264.7 cells with neutral red assay. Statistical significance was determined as *, p < 0.05 and **, p < 0.01 compared with the controls and represents the mean ± SD of triplicate tests. mHm: pcDNA3.1-HMBOX1 plasmid; Ve: pcDNA3.1 plasmid