Fig. 4

The lipid metabolism-related gene programs are activated by miR-149-5p overexpression. Porcine IM preadipocytes were collected 8 days after treatment, RNA was extracted, and RNA-seq was prepared. A Volcano plot of all measured genes’ pairwise comparisons. B The heatmap showed the relative expression of differential genes in RNA-seq. C Significantly up-regulated genes were examined by using KEGG pathway enrichment scatter plot statistical analysis. D Significantly down-regulated genes were examined by using KEGG pathway enrichment scatter plot statistical analysis. E Significantly up-regulated genes were examined by using GO pathway enrichment scatter plot statistical analysis. F Significantly down-regulated genes were examined by using GO pathway enrichment scatter plot statistical analysis