Fig. 7

MiR-149-5p targets ATP7A and affects IMF formation. A Schematic construction of ATP7A 3′ UTR dual-luciferase reporter vector. B Dual-luciferase assay detected porcine ATP7A in 293T cells. C After injecting miR-149-5p mimics into the porcine IM preadipocytes, miR-149-5p expression was observed (n = 3). D After 8 day’s induction, ATP7A, CTR1, and CTR2 in the porcine IM preadipocytes were checked (n = 3). E After injecting miR-149-5p inhibitors into the porcine IM preadipocytes, miR-149-5p expression was observed (n = 3). F After 8 day’s induction, ATP7A, CTR1, and CTR2 in the porcine IM preadipocytes were checked (n = 3). G–I The relative expressions of ATP7A (G), CTR1 (H), and CTR2 (I) in the longissimus dorsi, leg muscles, and subcutaneous fat of Queshan Black pigs in comparison with Yorkshire (n = 3)