Fig. 2

Changes in chromatin accessibility suggest a reduction in potency at the non-permissive temperature. A, B Replicates are highly similar within temperature conditions, with the majority of peaks present in all four replicates. C The two temperatures share 58,251 regions of open chromatin but do not overlap completely. D Principal component analysis resolves the two temperatures on the first principal component. E Differential accessibility analysis identifies 5,055 differentially accessible regions, with 2,654 preferentially open in the permissive temperature (37 °C) and 2,401 preferentially accessible at the non-permissive temperature (39 °C). F Gene ontology (GO) of genes adjacent regions that are preferentially open at the permissive temperature are associated with regulation of the cell cycle and negative regulation of differentiation, as is appropriate for this temperature. G Gene ontology of genes adjacent regions that are preferentially open at the non-permissive temperature are associated with a variety of differentiation fates (blood vessels, muscle cells, cartilage/chondrocytes). Additionally, two of the top gene ontology terms relate to the p38 MAPK cascade, which has been found to be activated as a cellular response to heat stress