Fig. 4

Gene Ontology and Differential Expression Analysis of Bulk RNA-seq Data: A Top 10 GO terms for downregulated DE genes in SN4741 cells at the non-permissive temperature, followed by GO terms of interest (below dotted line). Terms were evaluated using Combined Score Ranking = (p-value computed using the Fisher exact test)*(z-score computed by assessing the deviation from the expected rank), based on enrichment of DE genes that overlap with Enrichr input genes for each term (the end of each bar). B Top 10 predicted cell types based on downregulated DE genes in SN4741 cells at the non-permissive temperature, followed by predicted cell types of interest (below dotted line). Terms were evaluated using Combined Score Ranking = (p-value computed using the Fisher exact test)*(z-score computed by assessing the deviation from the expected rank), based on enrichment of DE genes that overlap with PanglaoDB input genes for each term (the end of each bar). C Top 10 GO terms for downregulated DE genes in SN4741 cells at the non-permissive temperature. D Top 10 predicted cell types based on upregulated DE genes in SN4741 cells at the non-permissive temperature. E Volcano plot of –log10 adjusted p-value versus log₂ fold change with DESeq2 after lfc shrinkage, contrasting the fold change in expression of SN4741 cells at 39 °C, using SN4741 cells at 37 °C as reference. Red points = genes that are statistically differentially expressed (adjusted p-value < 0.01, |log₂FC|> 1.5). Blue points = Overlapping immature neuron marker genes. F Blue points = Overlapping immature neuron marker genes. G Blue points = Overlapping oligodendrocyte marker genes. H Blue points = Overlapping DA neuron marker genes