Fig. 4

SNP analysis of the Fgf5 gene in rabbits. a SNPs in Fgf5. A total of 15 SNPs were detected in Fgf5, the loci of which are indicated in the schematic structure of the Fgf5 gene. b Alignment of the Fgf5 nucleotide sequences around the 19,234 and 20,857 loci. Fgf5 sequences of the eighteen rabbits were obtained from whole genome sequencing. The sequences of Angora (A1-A6), New Zealand (N1-N6) and Rex (R1-R6) are labelled with blue, red and green backgrounds, respectively. Dots indicate positions identical to the reference genome sequence (REF). Nucleotides inconsistent with the reference sequence are listed. The 19,234 and 20,857 loci of the Fgf5 gene are indicated using black triangles. T: thymine, C: cytosine, Y: thymine/cytosine. c Alignment of the FGF5 amino acid sequences around residue 191. Fgf5 coding sequences of the eighteen sequenced rabbits were translated into amino acid sequences, followed by sequence alignment. Dots indicate positions identical to the reference sequence (UniProtKB-G1T394). The missense mutations are shown. L: leucine, S: serine, X: leucine/serine. d Validation of the T19234C SNP by Sanger sequencing. EDTA-blood samples of 135 rabbits were collected from Shaanxi, Shandong, Jiangsu and Anhui provinces of China. The Fgf5 partial fragment was amplified, followed by sequencing and alignment analysis. For each province, nucleotide statistics of the 19,234 locus are presented in a pie chart