Fig. 10

Cellular models to verify expression of hub genes. (A) Morphological changes of PC12 and H9c2 cells before and after cell modeling. PC12 cells had reduced cell morphology and longer tentacles compared with normal cells after modeling. H9c2 cells had reduced cell morphology and fullness compared with normal cells after modeling. (B) Flow cytometry detection of apoptosis of PC12 and H9c2 cells before and after cell modeling. (C, D) CCK-8 assay of cell viability before and after modeling of PC12 and H9c2 cells showed a decrease in cell viability after modeling. (E, F) Significant apoptosis was observed after modeling of PC12 and H9c2 cells. (G) Immunofluorescence staining detected expression of key genes in PC12 and H9c2 cells and hypoxia-reoxygenated cell models (bar = 50 μm). (H, I) Expression levels of IL1B, FOS, JUN, FCGR2A, and SRC genes were significantly higher in PC12 cells after modeling compared with controls, and differences were statistically significant. Expression levels of FOS, IL1B, FCGR2A, and SRC were significantly elevated after modeling of H9c2 cells, and the difference was statistically significant, whereas difference in JUN expression was not. Nuclei were stained blue with DAPI. ^nsp>0.05; *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001