Table 5 Primer sequences
Primer | Primer sequences (5’-3’) | Product length(bp) | Annealing temperature(℃) |
|---|---|---|---|
IL1B F | ATGATGGCTTATTACAGTGGCAA | 132 | 60 |
IL1B R | GTCGGAGATTCGTAGCTGGA | ||
FOS F | GGGGCAAGGTGGAACAGTTAT | 126 | 60 |
FOS R | CCGCTTGGAGTGTATCAGTCA | ||
JUN F | CGCAAACCTCAGCAACTT | 248 | 60 |
JUN R | TCCGCTCCTGGGACTCCA | ||
FCGR2A F | TTTGAGATGAGTAATCCCAGCCA | 112 | 60 |
FCGR2A F | TCAGGCCCCAGTCTCCATTTTA | ||
SRC F | TGGCAAGATCACCAGACGG | 100 | 60 |
SRC R | GGCACCTTTCGTGGTCTCAC | ||
β-actin F | TGGCACCCAGCACAATGAA | 186 | 60.8 |
β-actin R | CTAAGTCATAGTCCGCCTAGAAGCA |