Fig. 2

Worker aggression is not associated with an increase in parent or lineage specific RNA m6A. Read abundance and m6A rate of parent and lineage alleles were assessed in non-aggressive and aggressive workers from an EHB and AHB reciprocal cross. The x-axis represents, for each transcript (1,928 in non-aggressive workers and 2,820 in aggressive workers), the average proportion of AHB reads that were methylated in workers with a EHB mother and AHB father, whereas the y-axis represents, for each transcript, the proportion of AHB reads that were methylated in workers with an AHB mother and EHB father. Each color represents a transcript which is significantly biased at all tested SNP positions: black is maternal, green is AHB, gold is EHB, blue is paternal, and gray is not significant. Significance was determined using an unpooled two-tailed z-test [63] along with previously established cutoff thresholds [61] of p1 < 0.4 and p2 > 0.6 for maternal bias, p1 > 0.6 and p2 < 0.4 for paternal bias, p1 < 0.4 and p2 < 0.4 for EHB bias, and p1 > 0.6 and p2 > 0.6 for AHB bias