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Fig. 3 | BMC Genomics

Fig. 3

From: Examining parent-of-origin effects on transcription and RNA methylation in mediating aggressive behavior in honey bees (Apis mellifera)

Fig. 3

Breeding scheme used to generate samples for experiments. In Block A, queens and drones were derived from one Africanized (AHB) honey bee colony and one European (EHB) colony. One queen and drone from each colony was mated to create a reciprocal cross pair (an EHB queen was mated with an AHB drone, and an AHB queens was mated with an EHB drone). The worker offspring of the two crosses in a pair were placed in a common colony and used for behavioral assays and subsequently collected for molecular analysis. In Block A, workers from reciprocal cross 1 was placed in Colony 1, and reciprocal cross 2 was placed in Colony 2. A similar mating scheme was used in Block B to generate Colony 3 and Colony 4. Molecular analysis was performed on the workers generated from reciprocal cross 2 (marked with a bracket)

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