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Fig. 5 | BMC Genomics

Fig. 5

From: Chimeras in Merlot grapevine revealed by phased assembly

Fig. 5

Bioinformatic workflow applied in this study. The workflow is described step by step. Step 1 is the kmers specific selection on each parent, these are variable size sequences that allow to specifically recognize reads from on parent. In step 2 these kmers are used to sort child reads out in two haplotypes that are each specific to one parent. If reads can’t be attributed to one parent, they are considered to be in both. Step 3 is the assembly of several reads into contigs for each haplotype. Step 4 is the building of pseudomolecules using multiple alignments. Step 5 is the transfer of annotation from the reference genome to the pseudomolecules

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