Fig. 4

P value, fold change, and transcript abundance of DEGs identified by bulk RNA-seq compound the effect of cell number on the identification of the DEGs by scRNA-seq. Effects of p value (from small to large), absolute log-transformed fold change (from large to small), and transcript abundance (from high to low) on the overlap of DEGs identified by bulk and pseudo-bulk RNA-seq (A) or scRNA-seq analyzed with Seurat with BH adjustment (B) or Bonferroni adjustment (C). 100% to 1% corresponded to scRNA-seq data from approximately 5,000 to 50 cells, randomly sampled three time at each level below 100%. DEG, differentially expressed gene; Log₂FC, log₂ fold change; TPM, transcript per million; Q1 to Q4, first to fourth quartile. N = 3