Fig. 5

Identification of DEGs with modest changes by scRNA-seq. DEGs with modest changes referred to the 1,437 DEGs identified from the bulk RNA-seq that had unadjusted p between 3.1 × 10^–5 and 0.025 (BH adjusted p values between 0.0001 and 0.05) and absolute log₂ fold changes between 0.5 and 2 (i.e., 1.4 to 4 fold). A Recapitulation of modest DEGs by pseudo-bulk RNA-seq. B Recapitulation of modest DEGs by scRNA-seq analyzed with Seurat with BH adjustment. C Recapitulation of modest DEGs by scRNA-seq analyzed with Seurat with Bonferroni adjustment. The bulk RNA-seq data were analyzed using BH adjustment. scRNA-seq analyzed using Bonferroni adjustment was plotted here for reference only as it was the default setting in Seurat. 100% to 1% corresponded to scRNA-seq data from approximately 5,000 to 50 cells, randomly sampled three time at each level below 100%. DEG, differentially expressed gene. N = 3