Fig. 7
From: Robustness of single-cell RNA-seq for identifying differentially expressed genes
DEGs identified from bulk RNA-seq with lower amounts of sequencing data. Down-samples of 40, 20, 10, and 5 million read pairs were close to the number of read pairs from 5,000, 2,000, 1,000, and 500 cells, respectively, in scRNA-seq. A Overlap of DEGs identified from various amounts of data and overlaps with DEGs from 100 M read pairs. B Effect of p value, fold change, and abundance on the overlap of DEGs identified by lower amounts of data with DEGs from 100 M read pairs. C Spearman correlation coefficients of p values of DEGs for 100 M read pairs vs. the down-samples. DEG, differentially expressed gene; log2FC, log2 fold change; TPM, transcript per million; Q1 to Q4, first to fourth quartile. N = 3