Fig. 7

Molecular cytogenetic analysis of Hyles euphorbiae chromosomes. Hybridization signals of the Cy3-labeled (TTAGG)_n telomeric probe (red) indicate chromosomal ends (a–c), and the fluorescein-labeled female gDNA probe (green) identifies the sex chromosome system (b and c) Chromosomes were stained with DAPI (grey). a) Male mitotic prometaphase stained with telomere-FISH showing a diploid chromosome number of 2n = 58. b) Male pachytene complement stained with a combination of GISH and telomere-FISH showing 29 bivalents, but without any bivalent highlighted, indicating a ZZ sex chromosome constitution. c) Female pachytene complement stained with a combination of GISH and telomere-FISH, showing 29 bivalents, including the WZ sex chromosome pair identified by the W chromosome highlighted with the female gDNA probe. Bar = 10 µm