Fig. 1

Early-life HFD induces fatty liver in medaka, which is reversed by subsequent NC. A Experimental design of early-life HFD feeding in medaka, where male fish were fed either HFD or NC for six weeks from hatching stage, followed by eight weeks of NC for both groups. B All fish were maintained individually to minimize variation of the speed of growth. C Body weight and liver weight at seven and 15 weeks of age. D Representative images of liver sections stained with Oil Red O (top right) and H&E (bottom), and E transmission electron microscopy (TEM) of liver sections from NC, HFD, NC-NC, and HFD-NC medaka. Scale bars: 100 µm in (D) and 5 µm in (E)