Fig. 3

Early-life HFD-induced changes in hepatocyte chromatin accessibility are largely reversed by subsequent NC. A A representative track view of RNA-seq, ATAC-seq, and ChIP-seq (H3K27ac, H3K27me3, and H3K9me3) signals for hepatocytes/livers for each of the dietary conditions. B Differentially accessible peaks in HFD fish hepatocytes compared to those in NC fish by ATAC-seq. X-axis: log2 fold change (HFD/NC) of normalized read counts within peaks, y-axis: –log10 p-value. C Gene ontology analysis of genes close to peaks with increased or decreased chromatin accessibility in HFD fish. Fold enrichment (orange) and –log10 FDR (bar) are displayed. D Motif analysis of peaks with increased (upper) and decreased (lower) accessibility, inferred by HOMER v4.11. E Differentially accessible peaks in HFD-NC fish hepatocytes compared to NC-NC fish. F Venn diagram of differentially accessible peaks at seven and 15 weeks of age. G Representative examples of ATAC-seq peaks at the promoters of fads2, srebf1, and cyp2r1 genes