Fig. 4

Early-life HFD-induced changes in liver histone modifications are largely reversed subsequent NC. A Differentially-H3K27ac enriched peaks in livers of HFD-fed fish compared to NC by ChIP-seq. X-axis: log2 fold change (HFD/NC) of normalized read counts within peaks, y-axis: –log10 p-value. B Differentially-H3K27ac-enriched peaks in livers of HFD-NC fish compared to NC-NC. C Overlaps of differentially-H3K27ac-enriched peaks at seven and 15 weeks of age. D Overlaps of differentially expressed, differentially accessible, and differentially-H3K27ac-enriched genes, at seven and 15 weeks of age. E Differentially-H3K27me3-enriched 4-kb bins in livers of HFD fish relative to NC (left) and HFD-NC relative to NC-NC (right). F Differentially-H3K9me3-enriched 4-kb bins in livers of HFD fish relative to NC (left) and HFD-NC relative to NC-NC (right). G Representative examples of reversible peaks (i.e., genomic regions where epigenetic states were changed soon after HFD feeding but were returned to normal levels by the following NC feeding). Left, fads2; right, s100a14