Fig. 5

Genes showing persistent changes in epigenetic state following long-term NC. A Gene ontology analysis of genes close to differentially accessible ATAC-seq peaks between HFD-NC and NC-NC fish (194 genes from 337 peaks). B A heatmap of persistent ATAC-seq peaks after reversal to NC (50 peaks in total). Log2-transformed, and Z-transformed, DESeq2 normalized read counts of ATAC-seq at each peak are displayed. DESeq2 results of RNA-seq and H3K27ac ChIP-seq of nearby genes/peaks are displayed on the right (p-value < 0.01). C, D Representative examples of ATAC-seq peaks showing persistent changes in chromatin accessibility, accompanied by long-term changes in expression of nearby genes (C) or gene expression that returned to normal level after a switch to NC (D)