Fig. 2

Effects of 15-day cold stress on routine metabolic rate, morphology of hepatopancreas, intestine, and intestine, and histology of hepatopancreas and muscle in G. przewalskii. A Resting metabolic rates of O₂ consumption (MO₂) were decreased by 4 °C environment. Data are shown as means ± SD. B & C Anatomical morphologies of hepatopancreas and intestine in 4 °C or 17 °C environment after a 15-day acclimation. Scale bar, 1 cm. (D&E) Histomorphology of the intestine stained with H&E at 17 °C (D) and 4 °C (E). The pinocytotic vesicle with a chyme particle center was labeled by a dark arrow. Scale bar, 50 µm. F–M Skeletal muscle (F–I) and hepatopancreas (J–M) stained with H&E (F, G, J, and K) or oil red O (H, I, L, and M). The gray arrow marked hyperchromatic cell nucleus and the dark arrow marked oil drops. Scale bar, 20 µm