Fig. 4

Gene regulatory differences between the prepuce cells of children and adults. A t-SNE plot of prepuce cells for children based on detected regulons. B t-SNE plot of prepuce cells for adults based on inferred regulons. C Expression profile of children-specific regulon AR. D Expression distribution of children-specific regulon KLF10. E–F Regulons with the same TF but having different sets of downstream target genes in children and adults. t-SNE plot showing the activation state of a regulon in cells (blue: activated; gray: un-activated). Venn plots displaying the intersection between the downstream target genes of the same TF-regulon in children and adults. The significantly enriched biological processes were shown in the bubble diagram