Fig. 4

Validating the expression of 5 differentially expressed miRNAs and 11 differentially expressed mRNAs by RT-qPCR. A The miRNA expression levels of miR-182-5p, miR-20a-5p, miR-17-3p were upregulated in CRCs, while the miR-139-5p was downregulated in CRCs. The mRNA expression levels of CHP2 and STC1 were upregulated, while FGL2 and CA1 were downregulated in CRCs. B Pearson’s correlation analysis of miRNA-mRNA regulatory pairs in 80 paired samples. Four negative correlated miRNA-mRNA regulatory pairs were plotted. Data are presented as mean ± SEM. *p.adj < 0.05, **p.adj < 0.01 and ***p.adj < 0.001 (Student's t-test). p-values are listed in Table 3