Fig. 1
Phylogenetic tree and pairwise comparisons based on both dDDH (d4) and ANI values of 64 genomes of Paenarthrobacter strains and of other Arthrobacter, Nocardioides, and Rhodococcus strains which possess the nic-genes. The tree was inferred with FastME 2.1.6.1 [33] from GBDP distances calculated from genome sequences. The branch lengths were scaled in terms of GBDP distance formula d5. Values in blue represent GBDP pseudo-bootstrap support values > 60% from 100 replications, with an average branch support of 67.3%. The tree was rooted at the midpoint. The same tree clustering was used for both columns and rows in the heatmap. Cut-off values for species clustering was 70% for dDDH (d4) [34] and 96% for ANI. Paenarthrobacter strains mislabelled as nicotinovorans are marked with a red asterisk. Strains previously reported to harbour nic-genes are marked with a black asterisk. Strains first reported here to possess the nic-genes are marked with a blue asterisk