Fig. 3

Nine highly syntenic nic-genes clusters identified in various Arthrobacter and Paenarthrobacter genomes. A BRIG comparative genomic analysis of the pAO1 megaplasmid against the strains harboring the nic-genes listed in the legend. External black circle – the localisation of the nic-genes cluster on pAO1; blue labels – pAO1 genes experimentally related to nicotine metabolism; red labels – genes related to recombination events. B Mauve alignments of the nic-genes clusters. Plasmids and genomes are listed on the left with the corresponding location of the nic-genes below. Coloured boxes – linear collinear blocks (LCB); white gaps – insertions and deletions; position atop or below the horizontal line represents the direction of LCB; red rectangles and text – recombination related ORFs; blue rectangles and text – pAO1 genes experimentally related to nicotine metabolism; nbr – nicotine blue oxidoreductase; mao – monoamine-oxidase; sad – succinic semi aldehyde dehydrogenase; folD—methylene-tetrahydrofolate dehydrogenase/ cyclohydrolase; abo—γ-N-methylaminobutyrate oxidase; purU—formyl-tetrahydrofolate deformylase; pmfR—transcriptional regulator; mobA—MobA, related to molybdopterin cytosine dinucleotide cofactor biosynthesis; nit—ω-amidase; hph—2,6-dihydroxypyridine-3-hydroxylase; pkc—putative polyketide cyclase; kdhL—ketone dehydrogenase, large subunit; pnh—2,6-dihydroxypseudooxynicotine hydrolase; kdhMS—ketone dehydrogenase, medium and subunits; 6hlno—6-hydroxy-L-nicotine oxidase; ndhLSM—nicotine dehydrogenase, large, small and medium subunits; moaA—molybdopterin cofactor synthesis protein; 6hdno—6-hydroxy-D-nicotine oxidase; hdnoR—transcriptional regulator; LCB2—locally colinear block containing genes associated with the synthesis of the molybdopterin cytosine dinucleotide cofactor; LCB1—locally colinear block containing genes for processing 6-hydroxy-D-nicotine. For an overview of the nicotine degradation pathway and role of each gene product, please see Supplementary Fig. 2