Fig. 3

Transcriptional changes of genes with H4K5acK8ac-preferred promoters upon JQ1 treatment. A Relative expression of genes upon JQ1 treatment. Box plots of gene expression fold changes (FCs) for 24-h JQ1 treatment relative to DMSO (vehicle control) are shown for H3K27ac-preferred (Groups 1–3) and H4K5acK8ac-preferred (Groups 4–6; see Methods) promoters (peaks within 1-kb of the transcription start site (TSS) and enhancers (peaks > 1-kb from the TSS) in 0316-GSC, U87, and C13NJ cells (n = 2 biological replicates for each histone modification and FC value of each gene is the average of the FCs of the two biological replicates). B Volcano plot showing the expression differences of H4K5acK8ac-preferred differentially regulated genes in 0316-GSC cells upon JQ1 treatment. The x and y-axes show log₂FC values and log₁₀ of the false discovery rate (FDR) values, respectively. A combination of thresholds for log₂FC values and for − log₁₀FDR values are used to classify the genes as significant. Black dots represent genes that were not statistically significant