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Fig. 6 | BMC Genomics

Fig. 6

From: Defining super-enhancers by highly ranked histone H4 multi-acetylation levels identifies transcription factors associated with glioblastoma stem-like properties

Fig. 6

Disruption of glioblastoma stem-like properties by siRNA knockdown of genes with H4K5acK8ac-preferred super-enhancers. A–C Disruption of genes by siRNA knockdown. A siRNA knockdown of MYCN and NFIC compared with the negative control (si-NC) in 0316-GSC cells (n = 3). B Short-term proliferation assay of 0316-GSC cells subjected to siRNA knockdown. Cell proliferation rates at 7 days after siRNA knockdown of MYCN and NFIC are shown (n = 6). C Expression of stem cell marker genes, NESTIN (left) and SOX2 (right), following siRNA knockdown of MYCN and NFIC (n = 3). D Sphere formation assay. Phase-contrast images of 0316-GSC cells treated with si-MYCN, si-NFIC, or si-NC for 2 weeks. Images are representative of three independent experiments. Scale bar, 50 μm. E Quantitation of sphere formation results for 0316-GSC cells (n = 3). A–C and E Data are means ± SEM **P < 0.01 (two-tailed Student’s t-test)

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BMC Genomics

ISSN: 1471-2164

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