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Table 2 Sequencing statistics across all samples

From: Large-scale analysis of sheep rumen metagenome profiles captured by reduced representation sequencing reveals individual profiles are influenced by the environment and genetics of the host

Metric

Parameter

Mean ± SD

Post-QC sequencinga

Reads per sample (thousands)

713 ± 215

Average read length (bp)

80.8 ± 1.5

Median read length

91.6 ± 0.5

Percent of reads ≥ 65 bp

79.3 ± 3.3%

Reference-based

Assignment rateb

20.8 ± 3.5%

Reference-free

Number of tagsc (thousands)

186

Assignment ratec

29.2 ± 3.8%

  1. aAfter demultiplexing and trimming; QC: Phred quality score of at least 20 and read length of at least 40 bp
  2. bPercent of reads that were assigned to the reference database, consisting of the Hungate1000 Collection plus four Quinella genomes
  3. cPercent of reads that were assigned to a tag, i.e., unique 65 bp reads, starting from the cut site, that are present in at least 25% of all samples
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BMC Genomics

ISSN: 1471-2164

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