Fig. 1

Locations of DMCpNs within the mitochondrial genome. The solar plot displays the results of our differential methylation analysis, showing the significance of differential methylation for CpN dinucleotides across the mitochondrial genome for each of our pairwise statistical comparisons; Male control vs. female control (MC vs. FC), male stress vs. female stress (MS vs. FS), male control vs. male stress (MC vs. MS) and female control vs. female stress (FC vs. FS). Each data point represents a possible differentially methylated CpN dinucleotide and is positioned based on its base pair (BP) location on the mitochondrial genome and its significance (-log(p-value)). Dashed lines indicate p-values of 0.05 and 0.005. The shaded blue peak area represents an example of an mtDMR within the COX1 gene, in which CpN methylation is clustered