Fig. 5

Applying DARIC to delineating compartment changes during cardiomyocyte differentiation. A Flow charts and pie charts comparing the genomic coverage of significantly changed regions in compartmentation revealed by DARIC and PC1-based switching analysis. B Stacked bar plot showing the percentage of genomic regions undergoing significant compartment changes during the cardiomyocyte differentiation. C Genome browser snapshots at the HAND2 and SOX2 locus showing compartment changes during differentiation and concordant epigenetic changes. D, E Bar plots showing the concordant gene expression changes for HAND2 (D) and SOX2 (E) during the differentiation. ***, p < 0.001, Wald test from DESeq2. F Pie chart representing the numbers of cardiomyocyte-specific genes which are associated or not associated with significant PIS increases. Cardiomyocyte-specific genes are defined as those significantly up-regulated in Day15 compared to Day00. G Gene and enhancer density comparison for the two gene sets as defined in (F). H An illustration showing the two mechanisms involved in the activation of cardiomyocytespecific genes during differentiation