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Fig. 2 | BMC Genomics

Fig. 2

From: Single-cell characterization of self-renewing primary trophoblast organoids as modeling of EVT differentiation and interactions with decidual natural killer cells

Fig. 2

Validation of the trophoblast organoids. (A) Images of hematoxylin staining for the cross-section of TO (Left) and first-trimester human placenta (Right). The red arrow indicates the intercellular lacunae. (B) Over-the-counter pregnancy test denoting “positive” for the culture medium containing TO growth before next passage. (C) Immunofluorescence staining images of GATA3, TFAP2A and TFAP2C in TO. (D) Quantification of the relative mRNA expression for ITGA6, VGLL1 and TP63 in TO, compared to first-trimester human placenta and decidua. (E) Quantification of the relative mRNA expression for HLA-A, -B, -C and -G in TO, compared to first-trimester human placenta and decidua. (F) qRT-PCR analysis on the expression of trophoblast specific microRNAs: miR517-5p, miR-526b-3p and miR525-3p in TO and first-trimester human placenta. Decidua serves as a positive control. (G) Immunofluorescence staining images of HLA-G in EVT differentiation stages of TO. (H) Quantification of the relative mRNA expression for PRG2, ITGA5 and MMP2 in EVT-TO, compared to TO. **P < 0.01; ***P < 0.001; ns, not significant. N = 3 in triplicate

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BMC Genomics

ISSN: 1471-2164

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