Fig. 4

Trophoblasts differentiation trajectories identify a similar pattern of in vivo placentation. (A) Annotations query of the trophoblast clusters in the TO system by Seurat mapping analysis. Reference database: the in vivo single-cell dataset of placenta (E-MTAB-6701). (B) Pseudotime ordering of the trophoblast clusters indicate the EVT pathways in TO system. (C) Pseudotime kinetics of specific representative genes from the root of the trajectory to EVT (solid line) and STB (dashed line). (D) Functional enrichment annotations of the clustering branched genes. Left, heatmap showing the genes in trajectory from root to EVT or STB. Middle and Right, Bar plots showing the top annotated GO terms and KEGG terms in four hierarchically clustering genes sets, respectively