Fig. 1

-Fe whole root and epidermis specific transcriptional comparative analysis. A DEG counts found in 1) whole root data using control at 0 time point only (Set Red), 2) cell-specific epidermis data using control at 0 time point only (Set Blue), and 3) cell-specific epidermis data using controls at all 7 time points (Set Green). The first two show differentially expressed genes involved in stress response & development. The first captures average behavior over several cell types and the second shows epidermis only. The third shows differentially expressed genes associated with iron response in the epidermis. B Heatmap comparing DEG expression in whole root and epidermis using control at 0 time point only. Blue box shows genes with significantly different expression patterns. Blue indicates up-regulation, Red indicates down-regulation. C Heatmap of expression for 38 known iron homeostasis DEGs in whole root and epidermis data using 0-time point control showing earlier activation in the epidermis vs. whole root. D DEG Gene counts at each time point identified using epidermis data using a control at each time point. The largest number of DEGs is seen at 24 h. The second largest is seen at 6 h. E List of genes that are differentially expressed at all 7 time points including 3 metal transporters and 3 known regulators of -Fe. F Upset diagram comparing sets of differentially expressed (under -Fe in the epidermis) genes at each time point. G Upset diagram showing the subsets of differentially expressed (under -Fe in the epidermis) transcription factors at each time point