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Fig. 1 | BMC Genomics

Fig. 1

From: CRISPR/Cas9-based depletion of 16S ribosomal RNA improves library complexity of single-cell RNA-sequencing in planarians

Fig. 1

Planarian 16S rRNA is highly enriched in various scRNA-seq platforms. A, B Violin plots show the percentage of 16S UMIs (A) and 12S UMIs (B) across different published scRNA-seq datasets. C, D Violin plots show the number of genes (C) and UMIs (D) per cell. Note, SmartSeq2 does not implement UMI. The values graphed indicate total read counts for SmartSeq2. DropSeq.1–5: “head” datasets [19]; DropSeq.6:gfp(RNAi) [21]; SmartSeq2 [22]; SplitSeq.1–3: 0 days post-amputation (dpa), 1dpa and 2dpa [23]; SplitSeq.ACME [24]; 10X.1: post-pharyngeal wound fragments, 0 h [25]; 10X.2: X1 cells[20]

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BMC Genomics

ISSN: 1471-2164

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