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Fig. 3 | BMC Genomics

Fig. 3

From: CRISPR/Cas9-based depletion of 16S ribosomal RNA improves library complexity of single-cell RNA-sequencing in planarians

Fig. 3

DASH depletes 16S UMIs and enhances library complexity. A-C, Boxplots show the percentage of 16S UMIs (A), numbers of genes (B) and non-16S UMIs (C) per cell in three replicates before and after DASH treatment. The medians of the ratios in DASHed versus untreated are shown on the top. Each biological replicate (rep) is downsampled to 100 million reads. D-E Rarefaction analysis of library complexity comparing untreated and DASHed libraries. Medians of genes (D) and non-16S UMI per cell (E) are shown. Each dot represents a downsampled replicate with indicated total reads

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BMC Genomics

ISSN: 1471-2164

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