Fig. 4

Post-CRISPR amplification increases library complexity. A, B Boxplots showing the UMIs (A) and genes per cell (B) in libraries amplified with different numbers of PCR cycles post-CRISPR. The medians are labeled in the 50th percentile in the box. C, D Rarefaction analysis of library complexity comparing the libraries amplified with different numbers of PCR cycles post-CRISPR, showing the medians of genes (C) and non-16S UMIs per cell (D). Each dot represents a downsampled replicate with indicated total reads