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Table 1 Selected bacterial strains and their Illumina assembly statistics

From: A comparison of Oxford nanopore library strategies for bacterial genomics

Species (MASAE accession)

Gram

Phylum, Order, Family

Contigs

GC

N50 (bp)

Mbp

Serratia proteamaculans (CD3406)

-

Pseudomonadota;Enterobacterales;Yersiniaceae

96

54.8%

163,495

5.87

Serratia proteamaculans (EBP3064)

-

Pseudomonadota;Enterobacterales;Yersiniaceae

60

55.2%

273,789

5.46

Serratia fonticola (MIP2602)

-

Pseudomonadota;Enterobacterales;Yersiniaceae

124

53.6%

139,619

6.21

Morganella morganii (HIS2824)

-

Pseudomonadota;Enterobacterales;Morganellaceae

43

50.3%

257,021

4.14

Hafnia paralvei (MIP2461)

-

Pseudomonadota;Enterobacterales;Hafniaceae

68

48.0%

347,145

4.89

Photobacterium phosphoreum (MIP2473)

-

Pseudomonadota;Vibrionales;Vibrionaceae

73

39.5%

144,700

4.48

Shewanella baltica (SF1039)

-

Pseudomonadota;Alteromonadales;Shewanellaceae

88

46.3%

150,379

5.05

Pseudomonas fluorescens (SF1671)

-

Pseudomonadota;Pseudomonadales;Pseudomonadaceae

187

60.1%

82,654

7.58

Weeksellaceae sp. (MIP2422)

-

Bacteriodata;Flavobacteriales;Weeksellaceae

42

36.6%

158,725

3.27

Bacillus velezensis (SAF3325)

 + 

Bacillota;Bacillales;Bacillaceae

36

45.9%

326,966

4.14

Lactococcus piscium (SAF3333)

 + 

Bacillota;Lactobacillales;Streptococcaceae

68

38.5%

132,316

2.25

Carnobacterium maltaromaticum (SF2022)

 + 

Bacillota;Lactobacillales;Carnobacteriaceae

26

34.3%

591,805

3.46

  1. Species name and MASAE collection accession for the 12 bacterial strains tested for nanopore sequencing. Phylum, order and family affiliation according to NCBI taxonomy. The number of contigs produced from Illumina assembly with Unicycler (pipeline CELIA) and resulting GC content (%), N50 (in bp), and estimated genome size (Mbp), are provided as an appreciation of genome fragmentation and putative genomic complexity. Note that with Illumina assemblies, Pseudomonas fluorescens was the most fragmented genome and Carnobacterium maltaromaticum was the most contiguous one