A comparison of Oxford nanopore library strategies for bacterial genomics

BMC Genomics

Table 3 Flow cell output and tandem content per library strategy

	Reads			Tandem content
	Mapped	Unmapped	All	Mapped	Unmapped	All
LIG	6.06 M	0.46 M	6.52 M	1.09 M	0.14 M	1.23 M
LIG	33.62 Gbp	1.52 Gbp	35.14 Gbp	0.29 Gbp	0.03 Gbp	0.32 Gbp
PCR	1.57 M	5.34 M	6.92 M	0.54 M	3.79 M	4.33 M
PCR	4.79 Gbp	2.47 Gbp	7.27 Gbp	0.22 Gbp	1.41 Gbp	1.63 Gbp
TAG	1.88 M	0.27 M	2.15 M	0.51 M	0.09 M	0.60 M
TAG	11.72 Gbp	0.68 Gbp	12.40 Gbp	0.20 Gbp	0.08 Gbp	0.28 Gbp

Number of reads in millions (M), cumulative read length and cumulative tandem repeat length in Giga base pair (Gbp) for all reads from library runs LIG, PCR and TAG. The breakdown of all reads is also detailed as mapped and unmapped reads to curated genomes. Note the large number of unmapped reads (very low quality or artifactual reads) and their tandem content in the PCR strategy

ISSN: 1471-2164