A comparison of Oxford nanopore library strategies for bacterial genomics

BMC Genomics

Table 4 Mean channel output per run and tandem content

	Reads			Tandem content
	Mapped	Unmapped	All	Mapped	Unmapped	All
LIG	11,898 ± 4,014	903 ± 1,866	12,801 ± 3,927	2,141 ± 631	281 ± 590	2,422 ± 739
LIG	66.06 Mbp ± 19.67	2.98 Mbp ± 6.84	69.04 Mbp ± 18.79	0.56 Mbp ± 0.17	0.06 Mbp ± 0.09	0.62 Mbp ± 0.17
PCR	3,088 ± 1,817	10,499 ± 5,553	13,587 ± 7,207	1,062 ± 562	7,438 ± 3,865	8,500 ± 4,044
PCR	9.42 Mbp ± 5.51	4.86 Mbp ± 2.93	14.28 Mbp ± 7.53	0.43 Mbp ± 0.23	2.77 Mbp ± 1.44	3.21 Mbp ± 1.66
TAG	3,692 ± 1,580	537 ± 807	4,221 ± 1,703	1,003 ± 440	168 ± 291	1,169 ± 501
TAG	23.07 Mbp ± 9.93	1.34 Mbp ± 3.09	24.37 Mbp ± 9.95	0.38 Mbp ± 0.19	0.16 Mbp ± 0.16	0.55 Mbp ± 0.28

Average number of reads, cumulative read length and cumulative tandem length in Million base pair (Mbp) for all reads from library runs LIG, PCR and TAG. The breakdown of all reads is also detailed as mapped and unmapped reads to curated genomes. Averages are reported with their standard deviation to appreciate output variation per channel and libraries

ISSN: 1471-2164