Fig. 5

Single cell RNA-seq revealed a long-lasting, conserved role for miR-375 in regulating Zfp36l2 in intestinal stem cell population of adult mice. (A) UMAP of initial clustering analysis with intestinal epithelial cells of adult WT and whole body miR-375 knockout (375KO) mice. N = 2 mice per genotype; 2937 cells from WT and 3275 cell from 375KO after doublet removal and quality control filtering. (B) UMAP of cell type annotation with intestinal epithelial cells of adult WT and 375KO mice. (C) Dot plot of known cell type markers and Zfp36l2 across all cell types defined in the single cell RNA-seq dataset. (D) Violin plot showing Zfp36l2 most enriched in stem cell population. (E) Significant upregulation of Zip36l2 in stem cell population of 375KO compared to WT mice. *** Adjusted P value = 0.000279 and log2FC = 0.346 by differential expression analysis using MAST method [95]. (F) UMAP overlain with the normal expression of Zfp36l2 in WT and 375KO mice. UMAP, Uniform Manifold Approximation and Projection. IEL, Intraepithelial lymphocytes; EEC, enteroendocrine cell; TA, transient amplifying cell