Fig. 3
From: Single-nucleus transcriptome sequencing reveals hepatic cell atlas in pigs
Quality control of snRNA-seq. a Cells with gene numbers from 240 to 3,600 were retained. The cells with fewer than 240 genes are considered low quality, and the cells with more than 3,600 genes are likely to be two or more cells in one drop; b Cells with 16,000 UMIs were retained. The cells with UMI numbers more than 16,000 are likely two or more cells in one drop; c Cells with 10% mitochondrial gene expression were retained. The percentage of mitochondrial gene expression more than 10% in a single cell indicates poor cell state, which is not conducive to subsequent analysis to reflect the real cell condition; d Relationship between nUMI and nGene. The dots in different colors represent cells from different samples. X axis is the number of UMI and Y axis is the number of genes percentage. The number at the top of the figure is the Pearson correlation coefficient between the number of UMI and the number of genes/mitochondria percentage; e Relationship between nUMI and pMito. The dots in different colors represent cells from different samples. X axis is the number of UMI and Y axis is the percentage of mitochondria. The number at the top of the figure is the Pearson correlation coefficient between the number of UMI and the percentage of mitochondria; f Quantity comparison before and after cell quality control