Fig. 3

Features of significantly changing site-specific editing, including volcano plots, gene regions of editing sites, and pathway enrichment analysis results. Panel A: Volcano plot of magnitude of changes in editing rate vs log transformed p value (FDR adjusted) of changes in editing rate for MCMV vs control samples (see Supplementary File 5B). 21 sites see significant increases in editing, with none significantly decreasing, p_adj < 0.05 (shown in blue). Panel B: Volcano plot of magnitude of changes in editing rate vs log transformed p value (FDR adjusted) of changes in editing rate for ZIKV vs control samples in PRJNA487357 (see Supplementary File 7B). 12 sites see significant increases in editing, with none significantly decreasing, p_adj < 0.05 (shown in blue). Panel C: Volcano plot of magnitude of changes in editing rate vs log transformed p value (FDR adjusted) of changes in editing rate for ZIKV vs control samples in PRJNA358758 (see Supplementary File 8B). 137 sites see significant increases in editing, while 11 see significant decreases, p_adj < 0.05 (shown in blue). Panel D: Volcano plot of magnitude of changes in editing rate vs log transformed p value (FDR adjusted) of changes in editing rate for ZIKV PE243 vs control hiNPC samples (see Supplementary File 10B). 3 sites see significant increases in editing, while none see significant decreases, p_adj < 0.05 (shown in blue). Panel E: Volcano plot of magnitude of changes in editing rate vs log transformed p value (FDR adjusted) of changes in editing rate for ZIKV FSS13205 vs control samples (see Supplementary File 10B). 4 sites see significant increases in editing, while 1 sees a significant decrease, p_adj < 0.05 (shown in blue). Panel F: Volcano plot of magnitude of changes in editing rate vs log transformed p value (FDR adjusted) of changes in editing rate for ZIKV FSS13205 vs PE243 samples (see Supplementary File 10B). One site sees a significant decrease in editing, p_adj < 0.05 (shown in blue). Panels G-J: Pie chart of the gene regions of significantly changing editing sites in MCMV, ZIKV PRJNA487357, ZIKV PRJNA358758, and ZIKV hiNPC data, respectively. Panels K-L: Dot plots showing significantly enriched Reactome pathways for editing sites (FDR < 0.1) in MCMV and ZIKV PRJNA487357 samples, respectively. Color indicates FDR, size of dot indicates the number of sites per pathway, and X axis shows gene ratio of the pathway enriched. ZIKV PRJNA358758 did not have any significantly enriched pathways (FDR < 0.1) and was not plotted