Table 1 RNA measurements and sequencing summary on tissues grouped by euthanasia method and storage condition

Storage Condition

59

 LN₂

23

12

11

8

8

7

0

 RNAlater

36

18

18

8

8

8

12

Euthanasia Method

59

 Anoxia

30

12

18

8

8

8

6

 T61

29

11

18

8

8

7

6

Tissue Type

59

 Hypothalamus

16

8

8

8

8

 Liver

16

8

8

8

8

 Lungs

15

8

7

7

8

 Pituitary

12

6

6

0

12

Time of sampling (minutes)

59

7.75 (1.44)

7.98 (1.18)

7.51 (1.65)

7.80 (1.50)

7.71 (1.41)

6.67 (1.02)

8.67 (1.39)

8.00 (1.45)

7.63 (1.06)

RNA Concentration (ng/µl)

59

674.7 (379.6)

732.1 (390.9)

615.4 (364.9)

607.4 (372.6)

717.7 (383.0)

444.7 (263.4)

838.4 (437.8)

568.1 (289.1)

896.4 (335.6)

A260_230 ratio

59

1.99 (0.29)

2.02 (0.22)

1.96 (0.34)

1.98 (0.21)

2.00 (0.33)

2.02 (0.21)

1.91 (0.28)

1.92 (0.40)

2.16 (0.12)

A260_280 ratio

59

2.11 (0.02)

2.11 (0.03)

2.10 (0.02)

2.12 (0.02)

2.10 (0.02)

2.11 (0.03)

2.10 (0.02)

2.12 (0.02)

2.09 (0.02)

RIN^a

56

8.81 (1.15)

8.85 (1.00)

8.77 (1.32)

7.99 (1.40)

9.26 (0.66)

7.56 (1.22)

9.43 (0.61)

9.31 (0.86)

9.18 (0.33)

Reads (million)

59

2.89 (0.38)

2.82 (0.37)

2.97 (0.37)

2.90 (0.33)

2.89 (0.41)

2.99 (0.37)

2.79 (0.41)

2.85 (0.34)

2.96 (0.38)

Alignment (%)

59

84.86 (1.27)

84.81 (1.30)

84.92 (1.26)

84.56 (1.33)

85.06 (1.21)

84.05 (0.90)

85.93 (1.24)

84.29 (1.0)

85.25 (0.91)

Unique alignment (%)

59

71.5 (3.8)

71.2 (3.7)

71.7 (3.9)

69.9 (3.3)

72.5 (3.8)

67.5 (2.1)

71.4 (4.2)

74.1 (2.2)

73.5 (1.6)

Aligned multimapping (%)

59

13.4 (3.4)

13.6 (3.1)

13.2 (3.8)

14.7 (2.9)

12.6 (3.5)

16.5 (2.2)

14.6 (3.3)

10.2 (2.1)

11.8 (1.2)

Non-aligned (%)

59

15.14 (1.27)

15.19 (1.30)

15.08 (1.26)

15.44 (1.33)

14.94 (1.21)

15.95 (0.90)

14.07 (1.24)

15.71 (1.00)

14.75 (0.91)