Fig. 1
From: Whole genome-scale assessment of gene fitness of Novosphingobium aromaticavorans during spaceflight
Schematic of FPA assembly used to culture N. aromaticivorans in spaceflight. Chamber 1 contained 1.5 ml of 2XPYE with an additional 2Â cm of airspace and was capped on the non-plunger end by a gas exchange membrane. Chamber 2 contained 1 ml of N. aromaticivorans TnSeq library inoculum in M2 salts. Chamber 3 contained 1.25 ml of RNAprotect fixative. Chambers were mixed by pressing the terminal septum with a plunger. When an internal septum reached the bypass, the liquid from the upper chamber was transferred to the lower chamber and mixed by shaking by hand