Fig. 2

Assessment of viability during cold stowage and growth time in FPAs. (A) N. aromaticivorans TnSeq libraries were assessed for viability loss during storage at low temperatures. Cells were resuspended in carbon-deficient 1X M2 salts in FPAs and incubated at 2 ^oC, 4 ^oC and 6 ^oC for 21 days. One replicate was removed every 7 days. Cells were serially diluted and plated on PYE agar plates and CFU/ml was calculated. (B) N. aromaticivorans TnSeq libraries were cultured inside FPAs and CFU/ml was assessed over 5 days. The numbers in blue indicate number of growth generations at each time point. The number of generations of growth at the time of sample extraction for the analysis has been indicated in red