Fig. 4
Synonymous mutations enhancing translational efficiency of the ccdB gene. A ESCcdB values of synonymous mutants belonging to ‘H + D’ class (ESCcdB < 0.7 and ESRelE < 1). B Enrichment of A/T at the wobble base for all synonymous mutants belonging to the ‘H + D’ class (left panel) and for the subset lying in the N-terminal region (right panel). C Synonymous codon mutational pattern for mutations with substantially altered ESCcdB as a function of amino acid. Amino acid identity is shown at the circumference of the radar plot. Each circle represents the number of mutants (shown on the vertical axis) obtained for each amino acid, showing a hyperactive (left panel) or inactive (right panel) phenotype