Fig. 1

RNA sequencing of isolated populations of border cells reveals temporal changes in gene expression. (A-C) Representative egg chambers showing the patterns of slbo-mCD8:GFP expression in the border cell cluster prior to (A), during (B), and after migration (C). (D) Representative migrating border cell cluster showing the pattern of slbo-mCD8:GFP expression in individual border cells (expressing Eyes absent, Eya) and polar cells (FasIII-positive, marked with asterisks). (E) Schematic of how the mCD8:GFP-positive border cells were sorted and selected using mCD8-bound magnetic beads. RNA from isolated border cells was then prepared for RNA sequencing and downstream analyses. (F) Heatmap of all sequencing results, identifying significant differential expression for 1,729 transcripts from isolated border cells during their migration (EBSeq-HMM FDR < 0.05). Several border cell migration-related genes, along with their transcript number (“FBtr”), are highlighted. Scale bars represent 10 μm (A-C) or 5 μm (D). All significantly differentially expressed transcripts are shown in Supplemental Data 2. HiSeq 2500 graphic courtesy of Illumina, Inc