Fig. 2

Migration and development-related genes are temporally expressed during border cell migration. (A-H) Heatmaps of significantly differentially expressed transcripts (EBSeq-HMM FDR < 0.05), focused on subsets of migration-related (A-F) or developmentally related (G, H) gene categories. The primary literature or other databases were used to identify genes and GO terms (see Methods for details). Heatmaps represent differential expression in border cells at pre-, mid-, or post-migration. A z-score of 1 (shown in red) signifies up-regulation; a z-score of -1 (shown in blue) signifies down-regulation. (A-C) Differentially expressed transcripts known to be required or expressed in border cells during their migration (A, 496 transcripts, representing 136 unique genes), or shown to be significantly upregulated in border cells versus non-migratory follicle cells from two microarray studies, Borghese et al. [18] (B, 1145 transcripts representing 475 unique genes) and Wang et al. [19] (C, 853 transcripts, 297 unique genes). (D-H) Transcripts for migration-related (D-F) or developmentally-related (G, H) genes, including the actin cytoskeleton (D, 154 transcripts, 38 unique genes), adhesion (E, 147 transcripts, 46 unique genes), epithelial-to-mesenchymal transition (EMT; F, 191 transcripts, 66 unique genes), oogenesis (G, 625 transcripts, 184 unique genes), or transcription factors (H, 701 transcripts, 263 unique genes). All data are shown in Supplemental Data 3