Fig. 7

Expression patterns of genes in migrating border cells in vivo. Fluorescence RNA in situ hybridization patterns of differentially expressed genes in migrating border cells from the transcriptomics analysis in migrating border cells using data and images from the Dresden Ovary Table (DOT) [62, 63]. Representative images of stage 9 and/or stage 10 egg chambers were chosen for given genes of interest. RNA signal is in green and DAPI labels the nuclei in magenta. Anterior is to the left in all images. (A-F) RNA signal (green) is shown in pre-, mid-, and post- migratory border cell clusters. (G-L) RNA signal (green) is shown at one stage of migration. (A, B) Expression of slbo (A) and sn (B), two genes known to regulate border cell migration. (C-L) Expression of multiple genes identified from the transcriptome analyses with previously uncharacterized roles in border cell migration. (M) Table of genes found in the DOT, along with their annotated expression patterns in the ovary, based on all available images, and location within one of the significantly differentially co-expressed gene clusters (SigDE Cluster). Arrowheads mark the position of border cells. All data are shown in Supplemental Data 9